Dynamic aberration correction for multiharmonic microscopy.

Nicolas Olivier (1) , Delphine Débarre (2) , Emmanuel Beaurepaire (1)

1 LOB - Laboratoire d'optique et biosciences
2 LIPhy - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères]

We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2(2N+1) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.

Domaines: Optique [physics.optics]

Nicolas Olivier, Delphine Débarre, Emmanuel Beaurepaire. Dynamic aberration correction for multiharmonic microscopy.. Optics Letters, 2009, 34 (20), pp.3145-7. ⟨hal-00681948⟩