Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.

Edward J Botcherby (1) , Christopher W Smith (1) , Michael M Kohl (2) , Delphine Débarre (3) , Martin J Booth (1) , Rimas Juskaitis (1) , Ole Paulsen (2) , Tony Wilson (1)

1 Department of Engineering Science
2 Department of Physiology, Anatomy and Genetics
3 LIPhy - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères]

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

Domaines: Optique [physics.optics]; Neurosciences [q-bio.NC]

Edward J Botcherby, Christopher W Smith, Michael M Kohl, Delphine Débarre, Martin J Booth, et al.. Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109 (8), pp.2919-24. ⟨10.1073/pnas.1111662109⟩. ⟨hal-00681944⟩