A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

Guido M. Voets (1) , A.C. Fluit (1) , Jelle Scharringa (1) , James Cohen Stuart (1) , Maurine A. Leverstein-Van Hall (1, 2)

1 Department of Medical Microbiology
2 National Institute for Public Health and the Environment

Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.

Mots clés: Multiplex PCR; Extended-spectrum β-lactamases; Plasmid-mediated AmpC β-lactamases; OXA β-lactamases; Carbapenemases

Domaines: Microbiologie et Parasitologie

Guido M. Voets, A.C. Fluit, Jelle Scharringa, James Cohen Stuart, Maurine A. Leverstein-Van Hall. A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases. International Journal of Antimicrobial Agents, 2011, 37 (4), pp.356. ⟨10.1016/j.ijantimicag.2011.01.005⟩. ⟨hal-00679598⟩