Characterisation of clinical and food animal isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A

Lorena López-Cerero (1) , Pilar Egea (1) , Lara Serrano (1) , Dolores Navarro (1) , Azucena Mora (2) , Jorge Blanco (2) , Yohei Doi (3) , David L. Paterson (4) , Jesús Rodríguez-Baño (1, 5) , Alvaro Pascual (1, 6)

1 University Hospital Virgen Macarena
2 Faculty of Veterinary Science
3 Division of Infectious Diseases
4 UQ [All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations] - The University of Queensland
5 Internal Medicine Department
6 Universidad de Sevilla = University of Seville

Seven phylogroup A CTX-M-15-producing isolates recovered from clinical and meat samples were further characterised. All of them belonged to sequence type ST410. Only 2 of the 22 virulence genes investigated were detected. All isolates carried the gene encoding type 1 fimbriae, and five isolates harboured the gene encoding aerobactin siderophore. A group of five isolates showed 81.2% similarity by pulsed-field gel electrophoresis (PFGE), comprising three clinical isolates belonging to ONT:H9 and two food isolates belonging to O55:H9. Different I digestion patterns were observed for plasmids, but all of them belonged to IncFIB group and harboured associated with , , , and surrounded by an identical genetic environment. These findings showed the possibility of lateral gene transfer of as well as other antibiotic resistance determinants between low-virulence food and clinical isolates.

Mots clés: CTX-M-15; Phylogroup A; Meat; ST410

Domaines: Microbiologie et Parasitologie

Lorena López-Cerero, Pilar Egea, Lara Serrano, Dolores Navarro, Azucena Mora, et al.. Characterisation of clinical and food animal isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A. International Journal of Antimicrobial Agents, 2011, 37 (4), pp.365. ⟨10.1016/j.ijantimicag.2011.01.001⟩. ⟨hal-00679592⟩