The PML nuclear bodies-associated protein TTRAP regulates ribosome biogenesis in nucleolar cavities upon proteasome inhibition

Stefano Gustincich (1) , Sandra Vilotti (1) , Marta Biagioli (1) , Rossana Foti (1) , Marco Dal Ferro (1) , Zeno Scotto Lavina (1) , Licio Collavin (1) , Giannino del Sal (1) , Silvia Zucchelli (1)

1 Neurobiology

TRAF and TNF Receptor Associated Protein (TTRAP) is a multifunctional protein that can act in the nucleus as a 5'-tyrosyl DNA phosphodiesterase and in the cytoplasm as a regulator of cell signaling. In this paper we show that in response to proteasome inhibition TTRAP accumulates in nucleolar cavities in a promyelocytic leukemia protein (PML)-dependent fashion. In the nucleolus, TTRAP contributes to control levels of rRNA precursor and processing intermediates and this phenotype is independent from its 5'-tyrosyl DNA phosphodiesterase activity. Our findings suggest a previously unidentified function for TTRAP and nucleolar cavities in ribosome biogenesis under stress.

Mots clés: TTRAP; Nucleolus; PML; Proteasome inhibition; rRNA biogenesis

Domaines: Biologie cellulaire

Stefano Gustincich, Sandra Vilotti, Marta Biagioli, Rossana Foti, Marco Dal Ferro, et al.. The PML nuclear bodies-associated protein TTRAP regulates ribosome biogenesis in nucleolar cavities upon proteasome inhibition. Cell Death and Differentiation, 2011, ⟨10.1038/cdd.2011.118⟩. ⟨hal-00679585⟩