Notch signaling is necessary for adult, but not fetal, development of RORγt(+) innate lymphoid cells.

Cécilie Possot (1, 2) , Sandrine Schmutz (1) , Sylvestre Chea (1, 2) , Laurent Boucontet (1) , Anne Louise (3) , Ana Cumano (1) , Rachel Golub (1, 2)

1 Développement des Lymphocytes
2 UPD7 - Université Paris Diderot - Paris 7
3 Cytométrie (Plate-forme)

The transcription factor RORγt is required for the development of several innate lymphoid populations, such as lymphoid tissue-inducer cells (LTi cells) and cells that secrete interleukin 17 (IL-17) or IL-22. The progenitor cells as well as the developmental stages that lead to the emergence of RORγt(+) innate lymphoid cells (ILCs) remain undefined. Here we identify the chemokine receptor CXCR6 as an additional marker of the development of ILCs and show that common lymphoid progenitors lost B cell and T cell potential as they successively acquired expression of the integrin α(4)β(7) and CXCR6. Whereas fetal RORγt(+) cells matured in the fetal liver environment, adult bone marrow-derived RORγt(+) ILCs matured outside the bone marrow, in a Notch2-dependent manner. Therefore, fetal and adult environments influence the differentiation of RORγt(+) cells differently.

Domaines: Immunologie

Cécilie Possot, Sandrine Schmutz, Sylvestre Chea, Laurent Boucontet, Anne Louise, et al.. Notch signaling is necessary for adult, but not fetal, development of RORγt(+) innate lymphoid cells.. Nature Immunology, 2011, 12 (10), pp.949-58. ⟨10.1038/ni.2105⟩. ⟨pasteur-00627238⟩