Application of primary haemocyte culture of in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Seena Jose (1) , P. Jayesh (1) , A. Mohandas (1) , Rosamma Philip (2) , I. S. Bright Singh (1)

1 CUSAT - Cochin University of Science and Technology
2 Department of Marine Biology

Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos. Using MTT assay 12 h IC values calculated were 31.09 ± 16.27 μM and 5.52 ± 1.16 μM for cadmium chloride and mercuric chloride and 59.94 ± 52.30 mg l and 186.76 ± 77.00 mg l for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in > 60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.

Mots clés: Aquaculture; primary haemocyte culture; heavy metals; organophosphate pesticides; cytotoxicity; genotoxicity; MTT assay; Comet assay

Domaines: Sciences du Vivant [q-bio]

Seena Jose, P. Jayesh, A. Mohandas, Rosamma Philip, I. S. Bright Singh. Application of primary haemocyte culture of in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides. Marine Environmental Research, 2011, 71 (3), pp.169. ⟨10.1016/j.marenvres.2010.12.008⟩. ⟨hal-00673207⟩