Photopotential Imaging on Functionalized Surfaces Dedicated to Label-Free Detection of Biomolecular Interactions

A. Charrier (1) , Yann Chevolot (1) , .J.-P. Cloarec (1) , A. Goudot (1) , T. Géhin (1) , Clément Roux , V. Dugas (2) , S. Vidal (3) , J.-J. Vasseur (4) , F. Morvan (4) , E. Souteyrand (1)

1 INL - C&N - INL - Chimie et Nanobiotechnologies
2 SA - Sciences Analytiques
3 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
4 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]

We present a label-free biosensor capable to map local potential by photogenerating charge carriers in a field effect device. It consists in an amplitude-modulated laser beam scanning through an electrolyte/insulator/semiconductor structure at very low illumination around the equilibrium state of semiconductor. A proof of principle is demonstrated by depositing a self-assembled monolayer on the dielectric and by showing a clear photopotential change between naked and covered areas.

Mots clés: photogeneration; Label-free biosensor; electrochemical impedance; field effect; photopotential imaging

Domaines: Chimie organique

A. Charrier, Yann Chevolot, .J.-P. Cloarec, A. Goudot, T. Géhin, et al.. Photopotential Imaging on Functionalized Surfaces Dedicated to Label-Free Detection of Biomolecular Interactions. Proc. Eurosensors XXV, Sep 2011, Athènes, Greece. pp.932-935, ⟨10.1016/j.proeng.2011.12.229⟩. ⟨hal-00668683⟩