Oligosaccharides-Protein Interaction Study using Microarrays with DDI Immobilisation

A. Goudot (1) , J. Zhang (1) , G. Pourceau (2) , A. Meyer (2) , J.-P. Cloarec (1) , S. Rouanet (2) , S. Vidal (3) , J.-J. Vasseur (2) , F. Morvan (2) , Y. Chevolot (1) , E. Souteyrand (1)

1 INL - C&N - INL - Chimie et Nanobiotechnologies
2 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
3 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

Carbohydrate and glycoconjugates play a major role in key biological events such as cell-cell recognition, pathogenesis and inflammation[1]. As a consequence, there is a need to understand the structural parameters governing the recognition of carbohydrate by their receptors. Technologies for rapid monitoring and evaluating such interactions are of great importance to provide deep insights relevant to carbohydrate involving biological events. However, most conventional approaches are cumbersome and material and time consuming. Carbohydrate microarray technology is a promising approach for probing carbohydrate/protein interactions because it permits the simultaneous screening of a number of biological interactions with only minute amounts of material. Herein, we develop a strategy to probe different glycoconjugates (different spatial configuration, linker, geometry...) toward proteins in one time on a DNA Directed Immobilization (DDI) oligosaccharide biochip.

Mots clés: glycoarrays; Biochips; Glycomics; Oligosaccharides/Protein interactions; IC50; DDI

Domaines: Chimie organique

A. Goudot, J. Zhang, G. Pourceau, A. Meyer, J.-P. Cloarec, et al.. Oligosaccharides-Protein Interaction Study using Microarrays with DDI Immobilisation. Proc. Eurosensors XXV, Sep 2011, Athène, Greece. pp.1553-1556, ⟨10.1016/j.proeng.2011.12.384⟩. ⟨hal-00668316⟩