Oral immunization of mice with expressing the rotavirus VP8* protein

Jesús Rodríguez-Díaz (1, 2) , Rebeca Montava (1) , Rosa Viana (2) , Javier Buesa (1) , Gaspar Pérez-Martínez (2) , Vicente Monedero (2)

1 Departamento de Microbiología, Facultad de Medicina
2 Laboratorio de Bacterias Lácticas y Probióticos

The efficacy of recombinant as a delivery vehicle for a rotavirus antigen was evaluated in a mouse model. The rotavirus VP8* protein was expressed intracellularly and extracellularly in wild type and in an mutant deficient in alanine racemase activity, necessary for the synthesis of the cell-wall component -alanine. When the mucosal immune response was evaluated by measuring VP8*-specific IgA antibody in faeces, wild-type triggered a low IgA synthesis only when the secreting strain was used. In contrast, VP8*-specific IgA was detected in faeces of both groups of mice orally given the mutant expressing extracellular VP8* and intracellular VP8*, which reached levels similar to that obtained with the wild type secreting strain. However, oral administration of the recombinant strains did not induce serum IgG or IgA responses. cell-wall mutants may therefore provide certain advantages when low-antigenic proteins are expressed intracellularly. However, the low immune response obtained by using this antigen-bacterial host combination prompts to the use of new strains and vaccination protocols in order to develop acceptable rotavirus immunization levels.

Mots clés: Lactic acid bacteria; Cell-wall; Vaccine; Rotavirus

Domaines: Biotechnologie; Biotechnologies

Jesús Rodríguez-Díaz, Rebeca Montava, Rosa Viana, Javier Buesa, Gaspar Pérez-Martínez, et al.. Oral immunization of mice with expressing the rotavirus VP8* protein. Biotechnology Letters, 2011, 33 (6), pp.1169-1175. ⟨10.1007/s10529-011-0551-6⟩. ⟨hal-00667592⟩