2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: evaluation of the spin trapping properties

Gilles Olive (1) , Anne Mercier (1) , François Le Moigne (1) , Antal Rockenbauer (2) , Paul Tordo (1)

1 SREP - Structure et Réactivité des Espèces Paramagnétiques
2 Chemical Research Center

The 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide (EMPO), an easily prepared pyrroline-N-oxide has been tested as free radical scavenger. Spin adducts of superoxide, hydroxyl radical and other free radicals were characterized in phosphate buffer at pH 7.0 and 5.6. At pH 7 in phosphate buffer, the EMPO/O2-* spin adduct was estimated to be about 5 times more persistent than its DMPO analogue. Furthermore, its decay does not produce the EMPO/ HO* adduct.

Mots clés: spin trapping; superoxide; hydroxyl radical; nitrones

Domaines: Autre

Gilles Olive, Anne Mercier, François Le Moigne, Antal Rockenbauer, Paul Tordo. 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: evaluation of the spin trapping properties. Free Radical Biology and Medicine, 2000, 28 (3), pp.403-408. ⟨hal-00660680⟩