Two-Photon Calcium Imaging of Evoked Activity from L5 Somatosensory Neurons in vivo.

Wolfgang Mittmann (1) , Damian Wallace (1) , Uwe Czubayko (1) , Jan Thomas Herb (1) , Andreas T. Schaefer (1) , Loren Looger (1) , Winfried Denk (1) , Jason N D Kerr (1)

1 Network Imaging Group

Multiphoton imaging is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. Here we combine regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend multiphoton imaging of neuronal population activity into layer 5 of adult mouse somatosensory cortex. We show that this approach can be used to record and quantify spontaneous and sensory-evoked activity in populations of layer 5 neuronal somata located as much as 800µm below the pia. In addition, we show that RAMM can be used to simultaneously image activity from large (~80) populations of apical dendrites and follow these dendrites down to their somata of origin.

Mots clés: Technical report; Sensory Systems; Neurophysiology; Neuroimaging; imaging

Wolfgang Mittmann, Damian Wallace, Uwe Czubayko, Jan Thomas Herb, Andreas T. Schaefer, et al.. Two-Photon Calcium Imaging of Evoked Activity from L5 Somatosensory Neurons in vivo.. Nature Neuroscience, 2011, ⟨10.1038/nn.2879⟩. ⟨hal-00658166⟩