Modified UroVysion scoring criteria increase the urothelial carcinoma detection rate in case of equivocal urinary cytology
Résumé
Aim UroVysion is a four-target fluorescence in situ hybridisation (FISH) technique to detect urothelial carcinoma (UC) in urinary cytology. The aim of this retrospective study is to investigate the UC detection rate of a modified UroVysion test in patients with equivocal urinary cytology. The modification comprised the addition of a cytological pre-screening technique and different evaluation criteria. Methods & results A thin layer slide was prepared of the residual urine sample of 82 patients with equivocal urinary cytology, pre-stained and pre-screened to confirm the presence of atypical urothelial cells. The same slide was used for the UroVysion test and scored according to different evaluation criteria. Results were compared with the outcome of cystoscopic and histologic findings. UroVysion detected 68% of the UCs when the manufacturer's evaluation criteria were applied. In case of altered evaluation criteria, the sensitivity increased to 81% when at least one copy number change of a probe target was considered as a positive test. The specificity only decreased from 84% to 82%. Conclusions Our data suggest that the sensitivity of the UroVysion test can be increased with the addition of a cytological pre-screening technique prior to the UroVysion test and a modification of the UroVysion evaluation criteria.
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