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Article Dans Une Revue Molecular and Cellular Endocrinology Année : 2010

Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells

Résumé

Leptin is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy, promoting antiapoptotic and trophic effects. Leptin receptor is present in trophoblastic cells and leptin may fully activate signaling. We have previously implicated the RNA-binding protein Sam68 in leptin signal transduction in immune cells. In the present work we have studied the possible role of Sam68 in leptin receptor signaling in trophoblastic cells (JEG-3 cells). Leptin dose-dependently stimulated Sam68 phosphorylation in JEG-3 cells, as assessed by immunoprecipitation and immunoblot with anti-phosphotyrosine antibodies. As previously observed in other systems, tyrosine phosphorylation of Sam68 in response to leptin inhibits its RNA binding capacity. Besides, leptin stimulation dose-dependently increases Sam68 expression in JEG-3 cells, as assessed by quantitative PCR. Consistently, the amount of Sam68 protein is increased after 24h of leptin stimulation of trophoblastic cells. In order to study the possible role of Sam68 on leptin receptor synthesis, we employed antisense strategy to knockdown the expression of Sam68. We have found that a decrease in Sam68 expression leads to a decrease in leptin receptor amount in JEG-3 cells, as assessed both by quantitative PCR and immunoblot.

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hal-00654963 , version 1 (24-12-2011)

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Flora Sánchez-Jiménez, Antonio Pérez-Pérez, Carmen González-Yanes, Souad Najib, Cecilia L. Varone, et al.. Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells. Molecular and Cellular Endocrinology, 2010, 332 (1-2), pp.221. ⟨10.1016/j.mce.2010.10.014⟩. ⟨hal-00654963⟩

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