Detection and diversity of a putative novel heterogeneous polymorphic proline-glycine repeat (Pgr) protein in the footrot pathogen
Résumé
a Gram negative anaerobic bacterium, is the essential causative agent of footrot in sheep. Currently, depending on the clinical presentation in the field, footrot is described as benign or virulent; strains have also been classified as benign or virulent, but this designation is not always consistent with clinical disease. The aim of this study was to determine the diversity of the gene, which encodes a putative proline-glycine repeat protein (Pgr). The gene was present in all 100 isolates of that were examined and, based on sequence analysis had two variants, and . In , there were two coding tandem repeat regions, R1 and R2: different strains had variable numbers of repeats within these regions. The R1 and R2 were absent from . Both variants were present in strains from Australia, Sweden and the UK, however, only was detected in isolates from Western Australia. The gene was detected in from tissue samples from two flocks in the UK with virulent footrot and only from a flock with no virulent or benign footrot for >10 years. Bioinformatic analysis of the putative PgrA protein indicated that it contained a collagen-like cell surface anchor motif. These results suggest that the gene may be a useful molecular marker for epidemiological studies.
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