Comparison of the PapilloCheck assay with the Digene HC2 HPV DNA assay for the Detection of 13 High-Risk Human Papillomaviruses in Cervical and Anal Scrapes. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Medical Virology Année : 2011

Comparison of the PapilloCheck assay with the Digene HC2 HPV DNA assay for the Detection of 13 High-Risk Human Papillomaviruses in Cervical and Anal Scrapes.

Marie-Noelle Didelot
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Nathalie Boulle
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Audrey Damay
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Valérie Costes
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Michel Segondy
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Résumé

The PapilloCheck assay was compared with the Digene HC2 HPV DNA assay for the detection of 13 high-risk human papillomaviruses (HPV) in 240 samples, including 181 cervical scrapes and 59 anal scrapes. Overall, 75 (30.5 %) samples were positive by the Digene HC2 HPV DNA assay: 34 (18.8%) cervical scrapes and 41 (69.5%) anal scrapes. By considering only the 13 high-risk HPV types detected by the Digene HC2 HPV DNA assay, 66 (27.5%) samples were positive by the PapilloCheck assay: 27 (14.9 %) cervical scrapes and 39 (66.1 %) anal scrapes. Concordant results between the two assays were obtained for 225 (93.8%) samples with a Kappa coefficient value of 0.85, indicating an excellent agreement. By considering all the HPV types detectable by the PapilloCheck assay, the overall prevalence of HPV was 34.2% (82/240): 21.0% (38/181) in cervical scrapes and 74.6% (44/59) in anal scrapes. Among the samples positive by the PapilloCheck assay, a multiple HPV infection (2 to 9 HPV types) was identified in 43 of 82 (52.4%) samples, including 7 of 38 (18.4%) cervical samples, and 36 of 44 (81.8%) anal samples. The prevalence of high-risk HPV, as determined by the PapilloCheck assay, was 17.6% (36/205) in samples with normal cytology, 83.9% (26/31) in samples with low-grade squamous intraepithelial lesions or atypical squamous cells of undetermined significance, and 100% (4/4) in samples with high-grade squamous intraepithelial lesions. The results obtained indicate that the PapilloCheck assay may be considered as a reliable screening test for HPV detection and typing.

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Dates et versions

hal-00652150 , version 1 (15-12-2011)

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Marie-Noelle Didelot, Nathalie Boulle, Audrey Damay, Valérie Costes, Michel Segondy. Comparison of the PapilloCheck assay with the Digene HC2 HPV DNA assay for the Detection of 13 High-Risk Human Papillomaviruses in Cervical and Anal Scrapes.. Journal of Medical Virology, 2011, 83 (8), pp.1377. ⟨10.1002/jmv.22148⟩. ⟨hal-00652150⟩

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