Development of Atlantic cod () exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish

Sonnich Meier (1) , H. Craig Morton (1) , Gunnar Nyhammer (2) , Bjørn Einar Grøsvik (1, 2) , Valeri Makhotin (3) , Audrey Geffen (2) , Stepan Boitsov (1) , Karen Anita Kvestad (2) , Anneli Bohne-Kjersem (2) , Anders Goksøyr (2) , Arild Folkvord (2) , Jarle Klungsøyr (1) , Asbjørn Svardal (1, 4)

1 IMR - Institute of Marine Research [Bergen]
2 Department of Molecular Biology/Biology
3 Department of Ichthyology
4 Section for Pharmacology

Produced water (PW) contains numerous toxic compounds of natural origin, such as dispersed oil, metals, alkylphenols (APs), and polycyclic aromatic hydrocarbons (PAHs). In addition, PW also contains many different chemicals which have been added during the oil production process. In the study described here, cod were exposed to real PW collected from an oil production platform in the North Sea. This was done in order to best recreate the most realistic field-exposure regime in which fish will be affected by a wide range of chemicals. The biological effects found in this study therefore cannot be assigned to one group of chemicals alone, but are the result of exposure to the complex chemical mixture found in real PW. Since APs are well known to cause endocrine disruption in marine organisms, we focused our chemical analysis on APs in an attempt to better understand the long term effects of APs from PW on the biology of fish. In this study, cod were exposed to several concentrations of real PW and 17β-oestradiol (E), a natural oestrogen, at different developmental stages. Cod were exposed to PW either during the embryo and early larvae stage (up to 3 months of age) or during the early juvenile stage (from 3 - 6 months of age). Results showed that, in general, APs bioconcentrate in fish tissue in a dose and developmental stage dependent manner during PW exposure. However, juveniles appeared able to effectively metabolise the short chain APs. Importantly, PW exposure had no effect on embryo survival or hatching success. However, 1% PW clearly interfered with the development of normal larval pigmentation. After hatching most of the larvae exposed to 1% PW failed to begin feeding and died of starvation. This inability to feed may be linked to the increased incidence of jaw deformities seen in these larvae. In addition, cod exposed to 1% PW, had significantly higher levels of the biomarkers vitellogenin and CYP1A in plasma and liver, respectively. No similar effects were seen in cod exposed to either 0.1% or 0.01% PW.

Mots clés: Alkylphenols; Early lifestages; Embryo; Endocrine disruption; Cod; Larvae; Produced water; Pigmentation; Sex differentiation

Sonnich Meier, H. Craig Morton, Gunnar Nyhammer, Bjørn Einar Grøsvik, Valeri Makhotin, et al.. Development of Atlantic cod () exposed to produced water during early life stages: Effects on embryos, larvae, and juvenile fish. Marine Environmental Research, 2010, 70 (5), pp.383. ⟨10.1016/j.marenvres.2010.08.002⟩. ⟨hal-00631697⟩