Potential rates and environmental controls of denitrification and nitrous oxide production in a temperate urbanized estuary

Catarina Teixeira (1) , Catarina Magalhães (1) , Rui A.R. Boaventura (2) , Adriano A. Bordalo (1)

1 University of Porto
2 Department of Chemistry

Denitrification may play a major role in inorganic nitrogen removal from estuarine ecosystems, particularly in those subjected to increased nitrate and organic matter loads. The Douro estuary (NW Portugal) suffers from both problems: freshwater input of nitrate and organic load from untreated wastewater discharges. To assess how these factors might control sediment denitrification, a 12-month survey was designed. Denitrification potential and nitrous oxide (NO) production were measured at different locations using the slurry acetylene blockage technique. Denitrification rate ranged from 0.4 to 38 nmol N g h, increasing towards the river mouth following an urban pollution gradient. NO production, a powerful greenhouse gas implicated on the destruction of the ozone layer, was significantly related with sediment organic matter and accounted for 0.5 - 47 % of the N gases produced. Additional enrichment experiments were consistent with the results found in the environment, showing that sediments from the upper less urban stretch of the estuary, mostly sandy, respond positively to carbon and, inversely, in organic rich sediments from the lower estuary, the denitrification potential was limited by nitrate availability. The obtained results confirmed denitrification as an important process for the removal of nitrate in estuaries. The presence of wastewater discharges appears to stimulate nitrogen removal but also the production of NO, a powerful greenhouse gas, exacerbating the NO: N ratio and thus should be controlled.

Mots clés: Denitrification; nitrous oxide; nitrate; urban pollution; estuaries; sediments

Catarina Teixeira, Catarina Magalhães, Rui A.R. Boaventura, Adriano A. Bordalo. Potential rates and environmental controls of denitrification and nitrous oxide production in a temperate urbanized estuary. Marine Environmental Research, 2010, 70 (5), pp.336. ⟨10.1016/j.marenvres.2010.07.001⟩. ⟨hal-00631691⟩