Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive Immuno PCR

Patrick Diel (1) , Thorsten Schiffer (2) , Stephan Geisler (2) , Torsten Hertrampf (1) , Stephanie Mosler (1) , Sven Schulz (3) , Karl Florian Wintgens , Michael Adler (3)

1 Dept. of Molecular and Cellular Sports Medicine
2 German Sport University Cologne [Germany]
3 Chimera Biotec GmbH

Myostatin propeptide (MYOPRO) and follistatin (FOLLI) are potent myostatin inhibitors. In this study we analysed effects of training and androgens on MYOPRO and FOLLI concentrations in blood and skeletal muscle using Immuno PCR. Young healthy males performed either a 3-month endurance-training or a strength-training. Blood and biopsy samples were analysed. Training did not significantly affect MYOPRO and FOLLI concentrations in serum and muscle. To investigate whether total skeletal muscle mass may affect circulating MYOPRO and FOLLI levels, blood samples of tetraplegic patients, untrained volunteers and bodybuilders were analysed. MYOPRO was significantly increased exclusively in the bodybuilder group. In orchiectomized rats MYOPRO increased in blood and muscle after treatment with testosterone. In summary our data demonstrate that moderate training does not affect the concentrations of MYOPRO to FOLLI. In contrast androgen treatment results in a significant increase of MYOPRO in skeletal muscle and serum.

Mots clés: Immuno PCR; Myostatin; Myostatin propeptide; Follistatin; Doping; Myostatin Inhibitor; Androgens

Patrick Diel, Thorsten Schiffer, Stephan Geisler, Torsten Hertrampf, Stephanie Mosler, et al.. Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive Immuno PCR. Molecular and Cellular Endocrinology, 2010, 330 (1-2), pp.1. ⟨10.1016/j.mce.2010.08.015⟩. ⟨hal-00629933⟩