Biochip for astrobiological applications: Investigation of low energy protons effects on antibody performances

M. Baqué (1) , A. Le Postollec (2) , G. Coussot (1) , Thibault Moreau (1) , I. Desvignes (1) , S. Incerti (3) , M. Dobrijevic (2) , P. Moretto (3) , O. Vandenabeele-Trambouze (1)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 sSE 2011
3 IPCV - Interface Physique et Chimie pour le Vivant

ntibody-based micro-arrays instruments are very promising tools for the search for biomarkers in planetary exploration missions. Since such instruments have never been used in this context, it is important to test their resistance to space constraints. In particular, cosmic particles might be deleterious. In the present study, we have investigated the effect of low energy protons (2 MeV) on antibody performances with fluences levels much greater than expected for a typical mission to Mars. We show that these particles do not alter significantly the antibody recognition capability for both free (in solution) and grafted (covalently bound to the support) freeze-dried antibodies. Details of the freeze-dried drying process used to optimize antibody performances during our experiments are also presented.

Mots clés: Antibody-based microarrays; Cosmic radiation; Life-detection instrument; Planetary exploration

Domaines: Planétologie et astrophysique de la terre [astro-ph.EP]; Planétologie et astrophysique de la terre [astro-ph.EP]

M. Baqué, A. Le Postollec, G. Coussot, Thibault Moreau, I. Desvignes, et al.. Biochip for astrobiological applications: Investigation of low energy protons effects on antibody performances. Planetary and Space Science, 2011, 59 (13), pp.1490-1497. ⟨10.1016/j.pss.2011.06.009⟩. ⟨hal-00623423⟩