Novel regulation of PLCζ activity via its XY-linker
Résumé
The XY-linker region of somatic cell PLC-b, -g, -d and -e isoforms confers potent catalytic inhibition suggesting a common auto-regulatory role. Surprisingly, the sperm PLCz XY-linker does not mediate auto-inhibition. Unlike for somatic PLCs, absence of the PLCz XY-linker significantly diminishes both in vitro PIP2 hydrolysis and in vivo Ca2+ oscillation-inducing activity, revealing evidence for a novel PLCz enzymatic mechanism.
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