Real-time PCR for detection of the O25b-ST131 clone of and its CTX-M-15-like extended-spectrum β-lactamases

Hiran Dhanji (1) , Michel Doumith (1) , Olivier Clermont (2) , Erick Denamur (2) , Russell Hope (1) , David M. Livermore (1) , Neil Woodford (1)

1 Health Protection Agency
2 EEM - Ecologie et Evolution des Microorganismes

CTX-M-15 has become the most prevalent extended-spectrum β-lactamase among in many countries during the past decade. Its dominance partly reflects the dissemination of an O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect belonging to the ST131 clone and to identify .

Mots clés: Epidemic; LightCycler PCR; ESBL; Pandemic clone

Hiran Dhanji, Michel Doumith, Olivier Clermont, Erick Denamur, Russell Hope, et al.. Real-time PCR for detection of the O25b-ST131 clone of and its CTX-M-15-like extended-spectrum β-lactamases. International Journal of Antimicrobial Agents, 2010, 36 (4), pp.355. ⟨10.1016/j.ijantimicag.2010.06.007⟩. ⟨hal-00616231⟩