Production of poly(3-hydroxybutyrate--3-hydroxyvalerate) by from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate

Stanislav Obruca (1) , Ivana Marova (1) , Ondrej Snajdar (1) , Ludmila Mravcova (2) , Zdenek Svoboda (3)

1 Faculty of Chemistry, Department of Food Chemistry and Biotechnology
2 Faculty of Chemistry, Department of Chemistry and Technology of Environmental Protection
3 Research Institute of Brewing and Malting, Malting Institute Brno

Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l, respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l h, respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate--3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.

Mots clés: Cupriavidus necator; Polyhydroxyalkanoates; Propanol; Stress conditions; Waste edible oils

Stanislav Obruca, Ivana Marova, Ondrej Snajdar, Ludmila Mravcova, Zdenek Svoboda. Production of poly(3-hydroxybutyrate--3-hydroxyvalerate) by from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate. Biotechnology Letters, 2010, 32 (12), pp.1925-1932. ⟨10.1007/s10529-010-0376-8⟩. ⟨hal-00614504⟩