A novel, thermostable manganese-containing superoxide dismutase from

Ivanka Petrova Boyadzhieva (1) , Miroslava Atanasova (1, 2) , Elka Emanuilova (1)

1 Department of Extremophilic Bacteria
2 Department of Molecular Biology and Biotechnology

A new, thermostable superoxide dismutase (SOD) from M20, isolated from Bulgarian mineral springs, was purified 11-fold with 11% recovery of activity. From native PAGE and SDS-PAGE, the enzyme was composed of two subunits of 21.5 kDa each. The SOD was inhibited only by NaN, which suggested that this SOD is of the manganese superoxide dismutase type. The purified enzyme had maximum activity at pH 8 and 55°C. The half-life of the SOD was 10 min at 95°C.

Mots clés: Enzyme thermal stability; Manganese-containing superoxide dismutase; Purification

Ivanka Petrova Boyadzhieva, Miroslava Atanasova, Elka Emanuilova. A novel, thermostable manganese-containing superoxide dismutase from. Biotechnology Letters, 2010, 32 (12), pp.1893-1896. ⟨10.1007/s10529-010-0368-8⟩. ⟨hal-00614503⟩