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Article Dans Une Revue European Journal of Clinical Microbiology and Infectious Diseases Année : 2010

Detection and quantification of leptospires in urine of dogs: a maintenance host for the zoonotic disease leptospirosis

Résumé

Leptospirosis is a global zoonotic disease. Pathogenic species, the causative agent of leptospirosis, colonize the renal tubules of chronically infected maintenance hosts such as dogs, rats and cattle. Maintenance hosts typically remain clinically asymptomatic and shed leptospires into the environment via urine. In contrast, accidental hosts such as humans can suffer severe acute forms of the disease. Infection results from direct contact with infected urine or indirectly, through contaminated water sources. In this study, a quantitative real-time PCR specific for was designed to detect the urinary shedding of leptospires from dogs. The sensitivity and specificity of the assay was evaluated using both a panel of pathogenic species and clinical microbial isolates, and samples of urine collected from experimentally infected rats and non-infected controls. The lower limit of detection was approximately 3 genome equivalents per reaction. The assay was applied to canine urine samples collected from local dog sanctuaries and the University Veterinary Hospital (UVH) at University College Dublin. Of 525 canine urine samples assayed, 37 were positive, indicating a prevalence of urinary shedding of leptospires of 7.05%. These results highlight the need to provide effective canine vaccination strategies and raise public health awareness.

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Dates et versions

hal-00601516 , version 1 (18-06-2011)

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P. Rojas, A. M. Monahan, S. Schuller, I. S. Miller, B. K. Markey, et al.. Detection and quantification of leptospires in urine of dogs: a maintenance host for the zoonotic disease leptospirosis. European Journal of Clinical Microbiology and Infectious Diseases, 2010, 29 (10), pp.1305-1309. ⟨10.1007/s10096-010-0991-2⟩. ⟨hal-00601516⟩

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