CYTOTOXIC ACTION OF GANODERMA LUCIDUM ON INTERLEUKIN-3 DEPENDENT LYMPHOMA DA-1 CELLS: INVOLVEMENT OF APOPTOSIS PROTEINS

Eva Calviño (1) , Lucía Pajuelo (1) , Jon A. Ochoa de Eribe Casas (2) , José L. Manjón (3) , María Cristina Tejedor (1) , Angel Herráez (1) , Manuel Díez Alonso (4) , José C. Diez (1)

1 Dpto. Bioquímica y Biología Molecular
2 Dpto. Biología Vegetal
3 Biología Vegetal
4 Servicio de Cirugía

Aqueous extracts and a semipurified fraction obtained by methanol extraction and column chromatography were isolated from Ganoderma lucidum [Ganoderma lucidum (Curtis) P. Karst.; Ganodermataceae Donk] and their effects on interleukin 3-dependent lymphoma cells (DA-1) were studied. Cell viability was reduced by the action of unboiled aqueous extract and by the methanol-extracted column-chromatography semipurified fraction, producing DNA fragmentation in DA-1 cells. Treatments with aqueous extracts showed increments of Bax after 13 hours, increments of p53 and Mdm2 after 19 hours and reduction of these three proteins after 24 hours. Metanol-extracted semipurified fraction also induced increments of p53 and Mdm2 factors at 19 hours with reduction after 24 hours. The methanol-extracted column-chromatography semipurified fraction from Ganoderma lucidum produced minor changes in the level of Akt in treatments for 19 hours in DA-1 cells with slight reduction in the levels of NFkB-p65 factor. Both the unboiled aqueous extract and the methanol-extracted column-chromatography semipurified fraction produced cleavage of inactive caspase 3, as a clear indication of induction of apoptosis by compounds present in Ganoderma lucidum.

Mots clés: Life Sciences

Eva Calviño, Lucía Pajuelo, Jon A. Ochoa de Eribe Casas, José L. Manjón, María Cristina Tejedor, et al.. CYTOTOXIC ACTION OF GANODERMA LUCIDUM ON INTERLEUKIN-3 DEPENDENT LYMPHOMA DA-1 CELLS: INVOLVEMENT OF APOPTOSIS PROTEINS. Phytotherapy Research, 2010, 25 (1), pp.25. ⟨10.1002/ptr.3202⟩. ⟨hal-00599829⟩