Evaluation of a new method for the measurement of corneal thickness in eye bank posterior corneal lenticules using Anterior Segment Optical Coherence Tomography
Résumé
Aim: To preliminary evaluate the repeatability of central corneal thickness (CCT) measurements performed with Anterior Segment Optical Coherence Tomography (AS-OCT) on eye bank posterior corneal lenticules. Methods: Six donor lenticules were created with a 350 μm head microkeratome (Moria, Antony, France). All donor tissues were stored at 4°C in Eusol-C solution (AL.CHI.MI.A. S.r.l., Ponte S. Nicolò, PD, Italy). The CCT of each lenticule, maintained in the glass vial, was measured using a commercial AS-OCT instrument (Visante, Carl Zeiss Meditec, Dublin, CA, USA) and a special designed adaptor immediately and 4-, 24- and 48-hours after dissection. Immediately after AS-OCT, CCT values were measured with the ultrasound (US) pachymetry method used at the Eye Bank. Results: The mean donor cornea central thickness was 647 ± 36 μm and 660 ± 38 μm (P=0.001) as measured by AS-OCT and US respectively; immediately after dissection, CCT values of posterior lenticules were 235 ± 43 μm and 248 ± 44 μm respectively (P=0.001). No statistically significant changes in CCT values of donor lenticules were assessed over the period of 48 hours with both methods. There was a high level of agreement, evidenced by Bland-Altman analysis, between the two methods of pachymetry. Conclusion: AS-OCT, with the corneal tissue in the vial, revealed to be a repeatable and reliable method for measuring posterior donor lenticule central thickness. Lenticule CCT values measured with the investigational AS-OCT method were on average 10 m thinner than those measured with the established US method.
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