Expression of transient receptor potential vanilloid-1 (TRPV1) in urothelial cancers of human bladder: relation to clinico-pathological and molecular parameters.

Sara Caprodossi (1) , Cristina Kalogris (1) , Consuelo Amantini (1) , Federica Lambertucci (2) , Massimo Nabissi (1) , Maria Beatrice Morelli (1, 3) , Valerio Farfariello (1, 3) , Alessandra Filosa (2) , Maria Cristina Emiliozzi (2) , Gabriele Mammana (4) , Giorgio Santoni (1)

1 Department of Experimental Medicine and Public Health
2 Pathology and Cytodiagnostic Unit
3 Department of Experimental Medicine
4 Urology Operative Unit

Aims: The aim of this study was to evaluate the expression of transient receptor potential vanilloid type-1 chennel protein (TRPV1) in normal and tumor urothelial tissues; to correlate TRPV1 expression with clinico-pathological parameters and with disease-specific survival. Methods and Results: TRPV1 expression was analyzed in normal and tumor urothelial samples at both mRNA and protein levels by Real-Time PCR and immunohistochemistry, respectively. TRPV1 down-regulation was found in UC specimens, which correlates with tumor progression. Moreover, TRPV1 mRNA levels were associated to clinico-pathological parameters to asses the role of TRPV1 down-regulation as a negative prognostic factor for survival. Kaplan-Meier survival analysis demonstrated a significantly shorter survival in patients showing TRPV1 mRNA down-regulation. Multivariate Cox regression analysis further indicated that TRPV1 mRNA expression retained its significance as an independent risk factor. Conclusions: The progression of UC of human bladder is associated with a marked decrease of TRPV1 expression, with a progressive loss in high-grade muscle invasive UC. Down-regulation of TRPV1 mRNA expression may represent an independent negative prognostic factor for the survival probability of bladder cancer patients.

Mots clés: Medicine

Sara Caprodossi, Cristina Kalogris, Consuelo Amantini, Federica Lambertucci, Massimo Nabissi, et al.. Expression of transient receptor potential vanilloid-1 (TRPV1) in urothelial cancers of human bladder: relation to clinico-pathological and molecular parameters.. Histopathology, 2010, 57 (5), pp.744. ⟨10.1111/j.1365-2559.2010.03683.x⟩. ⟨hal-00593446⟩