A new method for the rapid identification of tetracycline residues in foods of animal origin - using the Premi®Test in combination with a metal ion chelation assay

Sara Lucy Stead (1) , Marianne Caldow (1) , Anisha Sharma (1) , Helen Mary Ashwin (1) , Angelique De-Rijk (2) , Jaques Stark (2)

1 Food Safety Group
2 DSM Food Specialties

A post-screening classification assay for tetracycline compounds has been developed and integrated into the previously reported optimised Premi®Test methodology (Stead et al. 2004 and Stead et al. 2005) The new post-antimicrobial screening assay is based on a metal ion chelation using calcium and sodium chloride and has been shown to be specific towards the tetracycline class. The assay is both cost-effective and complementary to the post-screening procedures that have previously been developed for the b-lactam and sulfonamide compounds. A validation study was conducted in accordance with 2002/657/EC (Commission Decision). The method is rugged and applicable to a range of tetracyclines of differing antimicrobial potencies over a wide concentration range. A blind trial was undertaken in which all antimicrobial residues in the unknown samples were successfully identified by the analyst following the integrated Premi®Test procedure for the classification of antimicrobial compounds.

Mots clés: Life Sciences

Sara Lucy Stead, Marianne Caldow, Anisha Sharma, Helen Mary Ashwin, Angelique De-Rijk, et al.. A new method for the rapid identification of tetracycline residues in foods of animal origin - using the Premi®Test in combination with a metal ion chelation assay. Food Additives and Contaminants, 2007, 24 (06), pp.583-589. ⟨10.1080/02652030601134525⟩. ⟨hal-00577533⟩