Solid-phase microextraction-liquid chromatography-mass spectrometry applied to the analysis of insecticides in honey

Cristina Blasco (1) , Guillermina Font (1) , Yolanda Pico (2)

1 Laboratori de Bromatologia i Toxicologia, Facultat de Farmàcia
2 Laboratori de Bromatologia i Toxicologia, Facultat de Farmàcia

An approach based on solid–phase microextraction-liquid chromatography-mass spectrometry (SPME-LC-MS) has been developed for determining 12 insecticides (bromophos ethyl, chlorpyrifos methyl, chlorpyrifos ethyl, diazinon, fenoxycarb, fonofos, phenthoate, phosalone, pirimiphos methyl, profenofos, pyrazophos, and temephos) in honey. The influence of several parameters in the efficiency of the SPME was systematically investigated. In optimal conditions, the developed procedure provided an excellent linearity (>0.990), detection and quantification limits (between 0.001 and 0.1 µg g-1 and between 0.005 and 0.5 µg g-1, respectively), and precision (<19 % at the quantification limits and from 6 to 14 % at ten times higher concentrations). However, recoveries are not so good ranging from 19 to 92 %. Honey samples contaminated with bromophos ethyl, diazinon, phonophos, pirimiphos ethyl, pyrazophos and temephos were found; estimated concentration were from 6.2 ± 1.2 to 19 ± 3 ng g-1.

Mots clés: Life Sciences

Cristina Blasco, Guillermina Font, Yolanda Pico. Solid-phase microextraction-liquid chromatography-mass spectrometry applied to the analysis of insecticides in honey. Food Additives and Contaminants, 2007, 25 (01), pp.59-69. ⟨10.1080/02652030701424529⟩. ⟨hal-00577522⟩