Application of a liquid-chromatography – tandem mass spectrometric multi-mycotoxin method to different raw cereals and evaluation of matrix effects

Rudolf Krska (1)

1 Christian Doppler Laboratory for Mycotoxin Research

A multi-analyte method for the liquid-chromatography – tandem mass spectrometric determination of mycotoxins in crude grain extracts without any clean-up has been applied to the analysis of spelt, rice and barley. Method performance characteristics were determined after spiking blank samples on multiple levels and were found to be comparable for all investigated matrices as concerns linearity (linear calibration functions were obtained for all analyte/matrix combinations except for moniliformin), precision (coefficient of variations < 6 %) and sensitivity. Matrix induced signal suppression/enhancement was studied in detail and varied significantly between the investigated matrices as well as between individual samples (relative standard deviation was as high as 40% within three rice varieties) and individual toxins. It was therefore concluded that a reliable quantitative analysis using matrix-matched calibration requires a careful consideration of the model matrix as it should match the investigated samples as close as possible.

Mots clés: Life Sciences

Rudolf Krska. Application of a liquid-chromatography – tandem mass spectrometric multi-mycotoxin method to different raw cereals and evaluation of matrix effects. Food Additives and Contaminants, 2007, 24 (10), pp.1184-1195. ⟨10.1080/02652030701510004⟩. ⟨hal-00577474⟩