Validation of an analytical method for the determination of Carbadox and Olaquindox in feedingstuffs by Liquid Chromatography coupled to UV and/or DAD
Résumé
The method performance characteristics of an analytical method based on high performance liquid chromatography (HPLC) for the detection of the banned growth promoters carbadox and olaquindox in feedingstuffs were assessed through a collaborative study. The relative standard deviation for repeatability (RSDr) ranged from 1.1 % to 5.5 % for carbadox and from 2.5 % to 6.2 % for olaquindox. The relative standard deviation for reproducibility (RSDR) varied between 6.4 % and 10.7 % for carbadox and between 12.8 % and 20.0 % for olaquindox. The HORRAT values were in all cases equal or below the critical value of 1.5. Moreover the trueness in all cases was between the acceptance limits of 80 % and 110 %. Consequently it can be concluded that the method is suitable as a quantitative method. When a diode array detector was coupled to the HPLC instrument, the method was also qualitatively examined in terms of sensitivity, which is the correct identification of the target analytes based on the obtained UV spectrum. All sensitivity values were equal to or higher than 94% for olaquindox and carbadox, suggesting therefore the extension of the HPLC method from quantitative to confirmatory status when a diode array detector is used.
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