Development and application of a simplified clean-up procedure for the determination of PCBs and PBDEs in horse fat by gas chromatography-tandem mass spectrometry

Caroline Naert (1) , Carlos van Peteghem (2)

1 Laboratory of Food Analysis
2 Laboratory of Food Analysis

For the determination of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in adipose tissue, a simplified clean-up procedure was developed in combination with gas chromatography tandem mass spectrometry (GC-MS/MS). Clean-up was performed by the successive application of a Mega Bond Elut® silica column and a Bond Elut® PCB column. Validation of the method was conducted according to EU Commission Decision 2002/657/EC. In order to evaluate the applicability of the developed method, 44 horse fat samples were analysed. The total PCB concentration (sum of PCBs 28, 52, 101, 118, 138, 153, 180) ranged from 5.35 to 140 ng g-1 lipid weight. The total PBDE concentration (sum of BDEs 28, 47, 99, 100, 153, 154, 183) ranged from below the decision limit to 6.34 ng g-1 lipid weight.

Mots clés: Life Sciences

Caroline Naert, Carlos van Peteghem. Development and application of a simplified clean-up procedure for the determination of PCBs and PBDEs in horse fat by gas chromatography-tandem mass spectrometry. Food Additives and Contaminants, 2007, 24 (09), pp.1018-1025. ⟨10.1080/02652030701344768⟩. ⟨hal-00577447⟩