Validation of the procedure for the determination of maduramicin in concentrates, premixes, and feeds by liquid chromatography

Gabrijela Tavcar-Kalcher (1) , Katarina Vrtac (2) , Anton Vengust (2)

1 Unit for Pathology of Animal Nutrition and Environmental Hygiene
2 Unit for Pathology of Animal Nutrition and Environmental Hygiene

The validation of the procedure for the determination of maduramicin in concentrates, premixes, and feeds consisting of the extraction of maduramicin from a sample, derivatisation with dansylhydrazine and liquid chromatography with UV detection was performed. The limit of detection (LOD) and limit of quantification (LOQ) were 0.4 mg kg-1 and 1.0 mg kg-1, respectively. The repeatability of measurements, represented by average differences between results of duplicate measurements were 5.9 % at the concentration level of 1 % (concentrate), 7.1 % at the concentration level of 1 g kg-1 (premix), and 11 % with the feed containing maduramicin with the nominal concentration of 5 mg kg-1 and feed spiked at the concentration level of 1 mg kg-1. The relative standard deviation for the within-laboratory reproducibility (RSDW) was 9.2 %, 16 %, 18 %, and 17 % at the concentration levels of 1 %, 1 g kg-1, 5 mg kg-1, and 1 mg kg-1, respectively. The determined measurement uncertainties were ±0.2 %, ± 0.3 g kg-1, ±1.9 mg kg-1, and ±0.3 mg kg-1 at the same concentration levels, respectively.

Mots clés: Life Sciences

Gabrijela Tavcar-Kalcher, Katarina Vrtac, Anton Vengust. Validation of the procedure for the determination of maduramicin in concentrates, premixes, and feeds by liquid chromatography. Food Additives and Contaminants, 2009, 26 (01), pp.63-67. ⟨10.1080/02652030802225973⟩. ⟨hal-00577404⟩