LC-MS/MS determination of phase II metabolites of the mycotoxin zearalenone in the model plant Arabidopsis thaliana

Franz Berthiller (1) , Ulrike Werner (2) , Michael Sulyok (1) , Rudolf Krska (1) , Marie-Theres Hauser (2) , Rainer Schuhmacher (1)

1 Christian Doppler Laboratory for Mycotoxin Research, Institute for Agrobiotechnology (IFA-Tulln)
2 Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology

The biotransformation products of Zearalenone (ZON), a Fusarium mycotoxin, were elucidated using the model plant Arabidopsis thaliana. After treatment of plant seedlings with 50µM ZON both the liquid media and the plant extracts were analysed with HPLC-MS/MS. An array of 17 different metabolites, most prominently glucosides, malonylglucosides, dihexose- and hexose-pentose-disaccharides of ZON, α-Zearalenol and β-Zearalenol were detected in the samples. Time courses for the different ZON-metabolites were recorded and give a closer insight into the metabolism kinetic. A scheme proposing the ZON metabolism in A. thaliana is given. The aspect of food safety regarding the (potential) occurrence of masked mycotoxins in agricultural commodities is discussed.

Mots clés: Life Sciences

Franz Berthiller, Ulrike Werner, Michael Sulyok, Rudolf Krska, Marie-Theres Hauser, et al.. LC-MS/MS determination of phase II metabolites of the mycotoxin zearalenone in the model plant Arabidopsis thaliana. Food Additives and Contaminants, 2006, 23 (11), pp.1194-1200. ⟨10.1080/02652030600778728⟩. ⟨hal-00577296⟩