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Article Dans Une Revue Biotechnology Letters Année : 2010

Metabolic analysis of elicited cell suspension cultures of L. by H-NMR spectroscopy

Résumé

L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with jasmonic acid (JA) and pectin as elicitors to evaluate their effect on metabolism from two cell lines using NMR spectroscopy and multivariate data analysis. According to principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), the chloroform extract of the pectin-treated cultures were more different than control and JA-treated cultures; but in the methanol/water extract the metabolome of the JA-treated cells showed clear differences with control and pectin-treated cultures. Tyrosol, an antioxidant metabolite, was detected in cannabis cell cultures. The tyrosol content increased after eliciting with JA.
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Dates et versions

hal-00576175 , version 1 (13-03-2011)

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Jaroslav Peč, Isvett Josefina Flores-Sanchez, Young Hae Choi, Robert Verpoorte. Metabolic analysis of elicited cell suspension cultures of L. by H-NMR spectroscopy. Biotechnology Letters, 2010, 32 (7), pp.935-941. ⟨10.1007/s10529-010-0225-9⟩. ⟨hal-00576175⟩

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