%0 Journal Article %T Multimicroscopic study of curcumin effect on fixed nonmalignant and cancerous mammalian epithelial cells %+ Laboratoire Charles Coulomb (L2C) %A Saab, Marie-Belle %A Estephan, Elias %A Bec, Nicole %A Larroque, Marion %A Aulombard, Roger %A Cloitre, Thierry %A Gergely, Csilla %< avec comité de lecture %@ 1864-063X %J Journal of Biophotonics %I Wiley %P 1-11 %8 2011 %D 2011 %R 10.1002/jbio.201000119 %Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Journal articles %X The morphology changes, in particular the organization of microtubules in mammalian nonmalignant HMEC 184A1 and cancerous MCF-7 cells during curcumin treat- ment have been investigated by utilizing multiphoton, fluorescence, and atomic force (AFM) microscopies. Fluorescence microscopy reveals formation of ring-like structures of microtubules circumscribing the nuclear area in HMEC 184A1 cells after treatment, while in MCF-7 cells, no important changes were observed. Topo- graphy analyses of fixed HMEC 184A1 and MCF-7 be- fore and after treatment with curcumin were performed using AFM and the effect of the employed cells' fixation method was investigated on MCF-7 cells. Due to its in- depth optical sectioning capacity multiphoton microscopy provided valuable complementary information on curcu- min's effect on both cells' types. Combining information provided by AFM and optical fluorescence and biphoton microscopes allows us to gain a better understanding of the cells and their curcumin-induced changes, especially for microtubules which are the main target of antitumor chemotherapy treatments. Our multimicroscopic study demonstrates that 6 h incubation with curcumin does not induce significant modifications in the interphase micro- tubules in the malignant MCF7cell, whereas it has meas- urable effects on those of the nonmalignant HMEC 184A1 cells, revealing also morphology modifications over the nuclear area of these cells. %G English %L hal-00574664 %U https://hal.science/hal-00574664 %~ CNRS %~ L2C %~ MIPS %~ UNIV-MONTPELLIER %~ UM-2015-2021