A Sensitive Gas Chromatographic-Mass Spectrometric (C-MS) Method for the Determination of Bisphenol A in Rice-Prepared Dishes
Résumé
Certain chemicals possess the potential to modulate endocrine systems, and thereby interfere with reproductive and developmental processes. Bisphenol A is suspected to be one of them. The compound is widely used as a plastic additive, lacquer, resin, or plastic and can usually be found in food samples. An accurate and reproducible gas chromatographic-mass spectrometric (GC-MS) method to detect and measure trace amounts of the compound in rice-prepared dishes samples is proposed. A solid-liquid extraction with acetonitrile was carried out in order to isolate and preconcentrate the analyte. Later, solvent was removed and a silylation step using N,O-bis(trimethylsilyl)trifluoro acetamide/pyridine (BSTFA/PYR) was carried out. The silylated compound was identified and quantified by GC-MS using a DB-5 MS column. Bisphenol F was used as a surrogate. The detection limit found was 2.0 ng/g while inter- and intra-day variability was under 6%. Due to the absence of reference materials, the method was validated using standard addition calibration and a recovery assay. Recoveries for spiked samples were over 90% and under 105%
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