Validation data for HPLC/FLD determinations of ochratoxin A (OTA) in red paprika and black pepper adopting a one step clean-up procedure

Monica Bononi (1) , Franco Gallone (2) , Fernando Tateo (3)

1 Di.Pro.Ve.
2 Agrobiolab Laboratories
3 Di.Pro.Ve.

Validation data concerning the determination of ochratoxin A (OTA) in two spice matrices, red paprika and black pepper were obtained for samples treated with a simplified single step clean-up column Mycosep® 229. Extracts of finely ground samples of red paprika and black pepper were prepared and applied to a Mycosep® 229 Ochra clean-up column. The purified extract was then subjected to HPLC/FLD analysis. Relative Repeatability Standard Deviation (RSDr) of the method was evaluated and the values result 11.8% for red paprika and 9.9% for black pepper. The limit of detection (LOD) value (3 times the noise) was estimated as corresponding to the response of an extract derived from a blank matrix (previously washed) and spiked to 1.0 µg kg-1. The limit of quantitation (LOQ) (3 times LOD) was 3.0 µg kg-1. The performance of the one step column clean-up procedure appears to be a suitable alternative to commonly used clean-up techniques and allows the precise determination of OTA in two complex matrices.

Mots clés: Life Sciences

Monica Bononi, Franco Gallone, Fernando Tateo. Validation data for HPLC/FLD determinations of ochratoxin A (OTA) in red paprika and black pepper adopting a one step clean-up procedure. Food Additives and Contaminants, 2009, 27 (02), pp.249-254. ⟨10.1080/19440040903384182⟩. ⟨hal-00572622⟩