Plasma levels and zymographic activities of matrix metalloproteinases 2 and 9 in type II diabetics with peripheral arterial disease

Salvatore Santo Signorelli (1) , Grazia Malaponte (2) , Massimo Libra (2) , Luigi Di Pino (1) , Gabriella Celotta (1) , Valentina Bevelacqua (2) , Marcello Petrina (2) , Giuseppina S Nicotra (2) , Manuela Indelicato (2) , Patrick M Navolanic (2) , Giuseppe Pennisi (1) , Maria Clorinda Mazzarino (2)

1 Department of Internal Medicine
2 Department of Biomedical Sciences

Deregulation of matrix metalloproteinases (MMPs) is an important factor contributing to the development of vascular lesions. Plasma levels and zymographic activities of MMP-2 and MMP-9 were investigated in type II diabetics with ( = 51) or without ( = 42) peripheral artery disease (PAD) and in normal volunteers ( = 23). Plasma MMP-2 levels were higher in type II diabetics with ( < 0.01) or without ( < 0.05) PAD in comparison with normal volunteers. Similarly, type II diabetics with ( < 0.0001) or without ( > 0.05) PAD had higher plasma MMP-9 levels than normal volunteers. Plasma zymographic activities of both MMP-2 and MMP-9 were positively correlated with their plasma levels. Plasma MMP-2 zymographic activity was higher in type II diabetics with PAD than type II diabetics without PAD ( > 0.05). Plasma MMP-9 zymographic activity was higher in type II diabetics with ( < 0.0001) or without ( < 0.0001) PAD in comparision with normal volunteers. Together, these results indicate that increased plasma levels and zymographic activities of MMP-2 and MMP-9 may contribute to PAD in type II diabetics. In particular, plasma MMP-9 may be a useful marker for the development of vascular disease in type II diabetics.

Mots clés: MMP-2; MMP-9; type II diabetes; vascular disease

Salvatore Santo Signorelli, Grazia Malaponte, Massimo Libra, Luigi Di Pino, Gabriella Celotta, et al.. Plasma levels and zymographic activities of matrix metalloproteinases 2 and 9 in type II diabetics with peripheral arterial disease. Vascular Medicine, 2005, 10 (1), pp.1-6. ⟨10.1191/1358863x05vm582oa⟩. ⟨hal-00572112⟩