Proteomic analysis of pollen and allergen profiling by an immunoproteomic approach

Marilisa Barranca (1) , Simona Fontana (1) , Simona Taverna (1) , Giovanni Duro (2) , Isabelle Zanella-Cleon (3) , Michel Becchi (3) , Giacomo Leo (1) , Riccardo Alessandro (1)

1 Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche
2 Istituto di Biomedicina ed Immunologia Molecolare “Alberto Monroy”
3 IBCP - Institut de biologie et chimie des protéines [Lyon]

pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from allergic patients we obtained a more detailed characterization of allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.

Mots clés: Allergens; Immunoproteome; Pollen

Marilisa Barranca, Simona Fontana, Simona Taverna, Giovanni Duro, Isabelle Zanella-Cleon, et al.. Proteomic analysis of pollen and allergen profiling by an immunoproteomic approach. Biotechnology Letters, 2009, 32 (4), pp.565-570. ⟨10.1007/s10529-009-0180-5⟩. ⟨hal-00568368⟩