Kinetics of hepatic phase I and II biotransformation reactions in 8 finfish species

Jaime Fernando González M. (1) , Renate Reimschuessel (2) , Badar Shaikh (2) , Andrew S. Kane (3)

1 School of Veterinary Medicine & Animal Science - Universidad Nacional de Colombia
2 Center for Veterinary Medicine – Food Drug Administration (USFDA)
3 College of Public Health & Health Professions – University of Florida

Hepatic microsomes and cytosols of channel catfish (), rainbow trout (), Atlantic salmon (), red tilapia (.), largemouth bass (), striped bass (), hybrid striped bass ( x ) , and bluegill () (n = 8) were used to study the kinetics of phase I (ECOD, EROD, PROD, BROD) and phase II (UDP-glucuronosyltransferase (UDPGT)-, sulfotransferase (ST)- and glutathione-s-transferase (GST)- mediated) reactions. The best catalytic efficiency for ECOD and GST activities was performed by channel catfish, Atlantic salmon, rainbow trout and tilapia. The highest EROD catalytic efficiency was for Atlantic salmon. None of the species had either PROD or BROD activities. Rainbow trout had very similar UDPGT catalytic efficiency to tilapia, channel catfish, Atlantic salmon, largemouth bass and bluegill. Sulfotransferase conjugation had no significant differences among the species. In summary, tilapia, channel catfish, Atlantic salmon and rainbow trout had the best biotranforming capabilities; striped bass, hybrid striped bass and bluegill were low metabolizers and largemouth bass shared some capabilities with both groups.

Mots clés: hepatic; phase I and II biotransformation reactions; finfish

Jaime Fernando González M., Renate Reimschuessel, Badar Shaikh, Andrew S. Kane. Kinetics of hepatic phase I and II biotransformation reactions in 8 finfish species. Marine Environmental Research, 2009, 67 (4-5), pp.183. ⟨10.1016/j.marenvres.2009.01.002⟩. ⟨hal-00563069⟩