Encapsulation of recombinant expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one

Martin Hucík (1) , Marek Bučko (2) , Peter Gemeiner (2) , Vladimír Štefuca (1) , Alica Vikartovská (2) , Marko D. Mihovilovič (3) , Florian Rudroff (3) , Naseem Iqbal (3) , Dušan Chorvát (4) , Igor Lacík (5)

1 Faculty of Chemical and Food Technology SUT
2 Department of Glycobiotechnology
3 Institute of Applied Synthetic Chemistry
4 International Laser Centre
5 Polymer Institute

Recombinant cells, over-expressing cyclopentanone monooxygenase activity, were immobilized in polyelectrolyte complex capsules, made of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl and NaCl. More than 90% of the cell viability was preserved during the encapsulation process. Moreover, the initial enzyme activity was fully maintained within encapsulated cells while it halved in free cells. Both encapsulated and free cells reached the end point of the Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one to 4,9-dioxabicyclo[4.2.1]non-7-en-3-one at the same time (48 h). Similarly, the enantiomeric excess above 94% was identical for encapsulated and free cells.

Mots clés: Baeyer–Villiger biooxidation; Cyclopentanone monooxygenase; Encapsulation; Immobilization; Poly(methylene-co-guanidine)

Martin Hucík, Marek Bučko, Peter Gemeiner, Vladimír Štefuca, Alica Vikartovská, et al.. Encapsulation of recombinant expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one. Biotechnology Letters, 2010, 32 (5), pp.675-680. ⟨10.1007/s10529-010-0203-2⟩. ⟨hal-00560682⟩