ABSENCE OF POLYMERASE CHAIN REACTION-DETECTABLE HUMAN PAPILLOMA VIRUS IN ERYTHROPLASIA OF QUEYRAT USING A COMPARATIVE CONTROL GROUP

Maria Rita Nasca (1) , Maria Concetta Potenza (2) , Laura Alessi (1) , Gianluca Paravizzini (3) , Giuseppe Micali (1)

1 Unict - Università degli studi di Catania = University of Catania
2 U.O.C. Dermatology Unit, University of Rome La Sapienza, Rome
3 Clinical Laboratories “Girlando & Paravizzini”, Catania

Objectives High risk Human Papillomavirus (HPV) types have been linked with genital carcinomas. However, the exact relation between HPV and Erythroplasia of Queyrat (EQ), e.g. in situ carcinoma of the glans and prepuce, is still unclear. Aim of this study was to detect the presence of lesional HPV DNA in patients affected by EQ. Methods Paraffin-embedded biopsies were obtained from the glans or inner foreskin of 11 adult uncircumcised patients (mean age, 67.7 years; range, 57-79 years) with EQ. An equal number of randomly selected uncircumcised healthy control patients underwent a single brush cytology smear of the penile mucosa. Biopsy specimens and brushings were then assayed by a highly sensitive two-step nested polymerase chain reaction (PCR) technique based on MY11/MY09 consensus primers and general GP5+/GP6+ PCR primers, followed by cycle sequencing. Statistical evaluation was performed using conditional logistic regression analysis. Results None of the EQ or control samples proved to be positive for the presence of HPV DNA. Conclusions Our findings do not support the hypothesis that there is a considerable risk of EQ development in patients with HPV infection. Prevalence of HPV infection in patients with EQ has been seldom investigated and available data are relatively scant and controversial. Therefore, the relationship between HPV infection and the risk of progression of EQ into SCC remains a matter of debate, and further investigations are needed in order to confirm the role of HPV in delineating this risk.

Mots clés: ANOGENITAL CANCER; HPV; PENILE CARCINOMA; SKIN DISEASE; VIROLOGY

Maria Rita Nasca, Maria Concetta Potenza, Laura Alessi, Gianluca Paravizzini, Giuseppe Micali. ABSENCE OF POLYMERASE CHAIN REACTION-DETECTABLE HUMAN PAPILLOMA VIRUS IN ERYTHROPLASIA OF QUEYRAT USING A COMPARATIVE CONTROL GROUP. Sexually Transmitted Infections, 2010, 86 (3), pp.199. ⟨10.1136/sti.2009.039230⟩. ⟨hal-00557459⟩