FLOW CYTOMETRIC DIFFERENTIATION OF AVIAN LEUKOCYTES AND ANALYSIS OF THEIR INTRACELLULAR CYTOKINE EXPRESSION

Sandra de Boever (1) , Siska Croubels , Kristel Demeyere , Bart Lambrecht , Patrick de Backer , Evelyne Meyer

1 Pharmacology, Toxicology and Biochemistry

A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the pro-inflammatory cytokines interleukin-1β and IL-6 was subsequently optimised on whole blood after in vitro stimulation with lipopolysaccharide from Escherichia coli strain O127:B8.

Mots clés: Life Sciences

Sandra de Boever, Siska Croubels, Kristel Demeyere, Bart Lambrecht, Patrick de Backer, et al.. FLOW CYTOMETRIC DIFFERENTIATION OF AVIAN LEUKOCYTES AND ANALYSIS OF THEIR INTRACELLULAR CYTOKINE EXPRESSION. Avian Pathology, 2010, 39 (01), pp.41-46. ⟨10.1080/03079450903473574⟩. ⟨hal-00557320⟩