Prevalence of determinants among extended-spectrum β-lactamase-positive Enterobacteriaceae clinical isolates in southern Stockholm, Sweden

Hong Fang (1) , Haihui Huang (1) , Yuejie Shi (1) , Göran Hedin (1) , Carl Erik Nord (1) , Måns Ullberg (1)

1 Department of Laboratory Medicine

Three hundred and nineteen extended-spectrum β-lactamase-positive Enterobacteriaceae clinical isolates were screened for genes. Twelve isolates were positive for , including one , two , three , one , one and four . No -positive strains were identified among the isolates recovered before 2006. The first -positive was detected from a patient in 2006. genes remained rare in (6/288; 2.1%), but appeared to be more prevalent in (4/25; 16%) and (2/3; 66.7%). All -positive isolates were resistant to nalidixic acid while presenting varied susceptibilities to fluoroquinolones. Isolates harbouring or were highly resistant to all the fluoroquinolones tested. Their high-level resistance is associated with multiple chromosomal substitutions in and . Alterations at codons Ser-83 and Asp-87 in GyrA and at codons Ser-80 and Glu-84 in ParC were observed in these isolates.

Mots clés: Enterobacteriaceae; ESBL; Resistance; Sweden

Hong Fang, Haihui Huang, Yuejie Shi, Göran Hedin, Carl Erik Nord, et al.. Prevalence of determinants among extended-spectrum β-lactamase-positive Enterobacteriaceae clinical isolates in southern Stockholm, Sweden. International Journal of Antimicrobial Agents, 2009, 34 (3), pp.268. ⟨10.1016/j.ijantimicag.2009.03.016⟩. ⟨hal-00556339⟩