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Article Dans Une Revue Molecular Microbiology Année : 2010

MicA sRNA links the PhoP regulon to cell envelope stress

Résumé

Numerous small RNAs regulators of gene expression exist in bacteria. A large class of them binds to the RNA chaperone Hfq and act by base-pairing interactions with their target-mRNA, thereby affecting their translation and/or stability. They often have multiple direct targets, some of which may be regulators themselves, and production of a single sRNA can therefore affect the expression of dozens of genes. We show in this study that the synthesis of the E. coli pleiotropic PhoPQ two-component system is repressed by MicA, a σE-dependent sRNA regulator of porin biogenesis. MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competiting with ribosome binding. Consequently, MicA down-regulates several members of the PhoPQ regulon. By linking PhoPQ to σE, our findings suggest that major cellular processes such as Mg2+ transport, virulence, LPS modification or resistance to antimicrobial peptides are modulated in response to envelope stress. In addition, we found that Hfq strongly affects the expression of phoP independently of MicA, raising the possibility that even more sRNAs, that remain to be identified, could regulate PhoPQ synthesis.

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Dates et versions

hal-00552628 , version 1 (06-01-2011)

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Audrey Coornaert, Alisa Lu, Pierre Mandin, Mathias Springer, Susan Gottesman, et al.. MicA sRNA links the PhoP regulon to cell envelope stress. Molecular Microbiology, 2010, 76 (2), pp.467. ⟨10.1111/j.1365-2958.2010.07115.x⟩. ⟨hal-00552628⟩

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