Automated Lab Scale Production of PVA/PEG-Enzyme Immobilisates

Nora C. Bieler (1) , Marion Bettina Ansorge-Schumacher (1) , Lasse Greiner (2)

1 Dept. of Enzyme Technology (TC4)
2 Technical Chemistry

Entrapment of biocatalysts by cryogelation is a gentle method to extend the scope of biocatalysis. To foster the use of this versatile method we devised an automated injector for the production of polyvinyl alcohol (PVA)/polyethylene glycol (PEG) beads. The device consists of a thermostated reservoir connected to a programmable injector nozzle and an agitated receiving bath for the droplets. This lab scale production unit yields up to 1500 beads with immobilised enzyme per minute with a narrow size distribution and good roundness.

Mots clés: Life Sciences

Nora C. Bieler, Marion Bettina Ansorge-Schumacher, Lasse Greiner. Automated Lab Scale Production of PVA/PEG-Enzyme Immobilisates. Biotechnology Journal, 2010, 5 (8), pp.881. ⟨10.1002/biot.201000070⟩. ⟨hal-00552350⟩