Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization

Ioannis V Pavlidis (1) , Kyriakos Tzafestas (1) , Haralambos Stamatis (1)

1 Biological Applications and Technologies

The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non-polar organic solvents or ionic liquids, exhibiting up to 4.4-fold higher esterification activity compared to water-in-oil microemulsion-based organogels. The immobilized lipases retain their activity for several hours at 70^oC, while their half life time is up to 25-fold higher compared to that observed in w/IL microemulsions. Fourier transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behaviour observed.

Mots clés: Life Sciences

Ioannis V Pavlidis, Kyriakos Tzafestas, Haralambos Stamatis. Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization. Biotechnology Journal, 2010, 5 (8), pp.805. ⟨10.1002/biot.201000052⟩. ⟨hal-00552348⟩