CHARACTERIZATION OF AN INTRAVENOUS LIPOPOLYSACCHARIDE INFLAMMATION MODEL IN BROILER CHICKENS

Sandra de Boever (1) , Siska Croubels (1) , Evelyne Meyer (1) , Stanislas Sys (2) , Rudi Beyaert (3) , Richard Ducatelle (4) , Patrick de Backer (1)

1 Pharmacology, Toxicology and Biochemistry
2 Internal medicine and clinical biology of large animals
3 Biomedical Molecular Biology
4 Pathology, Bacteriology, and Poultry Disease

Intravenous administration of lipopolysaccharide (LPS) from Escherichia coli O127:B8 at a dose of 1,500,000 units/kg BW evoked a hypothermic response followed by a fever phase in five week old broiler chickens. The hypothermic phase coincided with a severe decrease in blood pressure. We assume that this decrease in blood pressure is, at least partly, responsible for the hypothermic phase of the body temperature curve. LPS administration also caused a decrease in circulating white blood cells. The heterophils were predominantly sequestered in the lungs. In LPS-treated chickens, far more apoptotic leukocytes were present in the circulation, compared to control chickens. The molecular players responsible for the LPS-induced inflammatory response could be TL1A, IL-1β and IL-6, since a slight increase in their mRNA levels in white blood cells was already seen 1 hour after LPS administration. In accordance with these observations, the levels of secreted IL-6 were maximal 3 hours after LPS administration. These parameters characterize this iv E. coli LPS inflammation model in broiler chickens.

Mots clés: Life Sciences

Sandra de Boever, Siska Croubels, Evelyne Meyer, Stanislas Sys, Rudi Beyaert, et al.. CHARACTERIZATION OF AN INTRAVENOUS LIPOPOLYSACCHARIDE INFLAMMATION MODEL IN BROILER CHICKENS. Avian Pathology, 2009, 38 (05), pp.403-411. ⟨10.1080/03079450903190871⟩. ⟨hal-00540164⟩